Get Academic Help Instantly!

Bristol University E Coli Genetics Questions Discussion


  • Novobiocin is a potent inhibitor of DNA replication in intact Escherichia coli cells.Gellert et al. (1976) Proc. Natl. Acad. Sci. USA 73:4474-4478 studied the effects of novobiocin in a cell free replication system and showed that it inhibited plasmid replication by altering the superhelical tension of the DNA.This could be rescued by the addition of a single protein of ~375 kDa isolated from novobiocin resistant E.coli.

1) What is a “cell free replication system” and how is it possible to use one to study DNA replication in E.coli?

2) Describe the major similarities and differences between DNA replication in E.coli and eukaryotes.

3) Formulate a hypothesis for Gellert et al.’s observations. How would you testt it?


10% off for this assignment.

Our Prices Start at $11.99. As Our First Client, Use Coupon Code GET10 to claim 10% Discount This Month!!

Why US?

100% Confidentiality
100% Timely Delivery
100% Original Writing

100% Money Back

Cheap Essay Writing

Do my Assignment

Order custom essay

Sample Essays

Custom term paper

Write My Research Paper

Affiliate program

Custom essay

Research paper

Write my essay

Write my paper

Essay writers

Become a Freelance Writer

Contact us



Terms and conditions

Privacy policy

Proficient Concepts  is a professional writing service that provides original papers. Our products include academic papers of varying complexity and other personalized services, along with research materials for assistance purposes only. All the materials from our website should be used with proper references.